Adaptive dynamic range shift (ADRIFT) quantitative phase imaging

Abstract Quantitative phase imaging (QPI) with its high-contrast images of optical delay (OPD) maps is often used for label-free single-cell analysis. Contrary to other methods, sensitivity improvement has not been intensively explored because conventional QPI sensitive enough observe the surface roughness a substrate that restricts minimum measurable OPD. However, emerging techniques utilize, example, differential image analysis consecutive temporal frames, such as mid-infrared photothermal QPI, mitigate OPD limit by decoupling static contribution and allow measurement much smaller OPDs. Here, we propose demonstrate supersensitive an expanded dynamic range. It enabled adaptive range shift through combination wavefront shaping dark-field techniques. As proof-of-concept demonstration, show expansion (sensitivity improvement) factor 6.6 utility in improving QPI. This technique can also be applied wide-field scattering dynamically changing nanoscale objects inside outside biological cell without losing global cellular morphological information.